Comparison of yeast cell protein solubilization procedures for two-dimensional electrophoresis (Record no. 39422)

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control field 20250625122452.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-5064
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Title Comparison of yeast cell protein solubilization procedures for two-dimensional electrophoresis
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Volume/sequential designation Electrophoresis, 20, p.826-829, 1999
520 3# - SUMMARY, ETC.
Summary, etc. Three different procedures for the solubilization of yeast (S. cerevisiae)cell proteins were compared on the basis of the obtained two-dimensional (2-D)polypeptide patterns. Major emphasis was laid on minimizing handling steps, protein modification or degradation, and quantitative loss of high molecular mass proteins. The procedures employed were sonication, followed by (i)protein solubilization with "standard" lysis buffer (9 M urea, 2
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Harder, A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Wildgruber, R.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Nawrocki, A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Fey, S.J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Larsen, P.M.
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Uniform Resource Identifier <a href="https://drive.google.com/file/d/1gyWW6uPvKIXAv84s5fpXEbfVo4ZXhb7D/view?usp=drivesdk">https://drive.google.com/file/d/1gyWW6uPvKIXAv84s5fpXEbfVo4ZXhb7D/view?usp=drivesdk</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-5064 25.06.2025 25.06.2025 Documentos solicitados