Use of two-dimensional liquid fractionation for separation of proteins from cell lysates without the presence of methionine oxidation (Record no. 40012)

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control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625122503.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-5666
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245 10 - TITLE STATEMENT
Title Use of two-dimensional liquid fractionation for separation of proteins from cell lysates without the presence of methionine oxidation
490 0# - SERIES STATEMENT
Volume/sequential designation Journal of Chromatography A, 1053(1-2), p.133-142, 2004
520 3# - SUMMARY, ETC.
Summary, etc. A novel two-dimensional (2D)chromatographic method is developed to separate proteins from malignant breast cancer whole cell lysates. Protein mixtures are first separated according to their pI by chromatofocusing followed by an orthogonal non-porous reversed-phase separation. An important advantage of this 2D chromatographic method is that, unlike gel-based methods, it does not result in methionine oxidation. The lack of methionine oxidation during separation is demonstrated by the analysis of protein tryptic digests using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF)MS. Our novel 2D chromatographic method used in combination with on-target lightinduced methionine oxidation provides a means for studying methionine-containing peptides. Methionine residues in peptide sequences are partially oxidized with light exposure. Neither the location nor the modification of methionine in the peptide sequence affects the oxidation. As a result, multiple peaks are observed in MALDI-TOF-MS spectra after light exposure. Sequence information derived from light-induced methionine can be applied to enhance the database search results obtained through peptide mass fingerprinting.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element REVERSED-PHASE SEPARATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MALDI-TOF
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element 2D-LC
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element METHIONINE OXIDATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CHROMATOFOCUSING
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Zhu, K.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kachman, M.T.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Miller, F.R.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Lubman, D.M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Zand, R.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1ayhwq6YQvCGXMS2e6AwU-wt68WymeidI/view?usp=drivesdk">https://drive.google.com/file/d/1ayhwq6YQvCGXMS2e6AwU-wt68WymeidI/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-5666 25.06.2025 25.06.2025 Documentos solicitados