BIBAC and TAC clones containing potato genomic DNA fragments larger than 100 kb are not stable in Agrobacterium (Record no. 40109)

MARC details
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fixed length control field 02033nam a2200265Ia 4500
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control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625122504.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-5763
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245 10 - TITLE STATEMENT
Title BIBAC and TAC clones containing potato genomic DNA fragments larger than 100 kb are not stable in Agrobacterium
490 0# - SERIES STATEMENT
Volume/sequential designation Theoretical and Applied Genetics, 107(5), p.958-964, 2003
520 3# - SUMMARY, ETC.
Summary, etc. Development of efficient methods to transfer large DNA fragments into plants will greatly facilitate the map-based cloning of genes. The recently developed BIBAC and TAC vectors have shown potential to deliver large DNA fragments into plants via Agrobacterium-mediated transformation. Here we report that BIBAC and TAC clones containing potato genomic DNA fragments larger than 100 kb are not stable in Agrobacterium. We tested the possible factors that may cause instability, including the insert sizes of the BIBAC and TAC constructs, potato DNA fragments consisting of highly repetitive or largely single-copy DNA sequences, different Agrobacterium transformation methods and different Agrobacterium strains. The insert sizes of the potato BIBAC and TAC constructs were found to be critical to their stability in Agrobacterium. All constructs containing a potato DNA fragment larger than 100 kb were not stable in any of the four tested Agrobacterium strains, including two recA deficient strains. We developed a transposon-based technique that can be used to efficiently subclone a BAC insert into two to three BIBAC/TAC constructs to circumvent the instability problem.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element BAC
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element BIBAC
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TAC
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element AGROBACTERIUM
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PLANT TRANSFORMATION
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Song, J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Bradeen, J.M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Naess, S.K.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Helgeson, J.P.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/18_ggRNbhnoYRaC61He59av9CmC-if-pW/view?usp=drivesdk">https://drive.google.com/file/d/18_ggRNbhnoYRaC61He59av9CmC-if-pW/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-5763 25.06.2025 25.06.2025 Documentos solicitados