Construction of BAC libraries from two apomictic grasses to study the microcolinearity of their apospory-specific genomic regions (Record no. 40176)
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| fixed length control field | 02098nam a2200229Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625122506.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-5831 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Construction of BAC libraries from two apomictic grasses to study the microcolinearity of their apospory-specific genomic regions |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Theoretical and Applied Genetics, p.104, 2002 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | We have constructed bacterial artificial chromosome (BAC)libraries from two grass species that reproduce by apospory, a form of gametophytic apomixis. The library of an apomictic polyhaploid genotype (line MS228-20, with a 2C genome size of approximately 4,500 Mbp)derived from a cross between the obligate apomict, Pennisetum squamulatum, and pearl millet ( P. glaucum)comprises 118,272 clones with an average insert size of 82 kb. The library of buffelgrass ( Cenchrus ciliaris, apomictic line B-12-9, with a 2C genome size of approximately 3,000 Mbp)contains 68,736 clones with an average insert size of 109 kb. Based on the genome sizes of these two lines and correcting for the number for false-positive and organellar clones, library coverages were found to be 3.7 and 4.8 haploid genome equivalents for MS 228-20 and B12-9, respectively. Both libraries were screened by hybridization with six SCARs (sequence-characterized amplified regions), whose tight linkage in a single apospory-specific genomic region had been previously demonstrated in both species. Analysis of these BAC clones indicated that some of the SCAR markers are actually amplifying duplicated regions linked in coupling in both genomes and that restriction enzyme mapping will be necessary to sort out the duplications. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Roche, D. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Conner, J.A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Budiman, M.A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Frisch, D.&Wing, R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hanna, W.W. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ozias-Akins, P. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1N55JT_cyJx5FDRoBDcagTND2eQ7l6cXy/view?usp=drivesdk">https://drive.google.com/file/d/1N55JT_cyJx5FDRoBDcagTND2eQ7l6cXy/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-5831 | 25.06.2025 | 25.06.2025 | Documentos solicitados |