Transgenic plants of coffea canephora from embryogenic callus via agrobacterium tumefaciens-mediated transformation (Record no. 40330)

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control field MX-MdCICY
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control field 20250625122509.0
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Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-5985
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245 10 - TITLE STATEMENT
Title Transgenic plants of coffea canephora from embryogenic callus via agrobacterium tumefaciens-mediated transformation
490 0# - SERIES STATEMENT
Volume/sequential designation Plant Cell Reports, 19, p.106-110, 1999
520 3# - SUMMARY, ETC.
Summary, etc. Abstract Embryogenic calli were induced from leaf explants of coffee (Coffea canephora)on McCown's woody plant medium (WPM)supplemented with 5 7M N6-(2-isopentenyl)-adenosine (2-iP). These calli were co-cultured with Agrobacterium tumefaciens EHA101 harboring pIG121-Hm, containing #-glucuronidase (GUS), hygromycin phosphotransferase (HPT), and neomycin phosphotransferase II genes. Selection of putative transgenic callus was performed by gradual increase in hygromycin concentration (5, 50, 100 mg/l). The embryogenic calli surviving on medium containing 100 mg/l hygromycin showed a strong GUS-positive reaction with X-Gluc solution. Somatic embryos were formed from these putative transgenic calli and germinated on WPM medium with 5 7M 2-iP. Regenerated small plantlets with shoots and roots were transferred to medium containing both 100 mg/l hygromycin and 100 mg/l kanamycin for final selection of transgenic plants. The selected plantlets exhibited strong GUS activity in leaves and roots as indicated by a deep blue color. GUS and HPT genes were confirmed to be stably integrated into the genome of the coffee plants by the polymerase chain reaction.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element COFFEA CANEPHORA
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element AGROBACTERIUM TUMEFACIENS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TRANSFORMATION
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Hatanaka, T.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Choi, Y.E.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kusano, T.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Sano, H.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1oJ4ThleYakSaic7u2MbLHjcc-Wly_VSf/view?usp=drivesdk">https://drive.google.com/file/d/1oJ4ThleYakSaic7u2MbLHjcc-Wly_VSf/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-5985 25.06.2025 25.06.2025 Documentos solicitados