Cyclin A/Cdk2 complexes regulate activation of Cdk1 and Cdc25 phosphatases in human cells (Record no. 40365)

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control field MX-MdCICY
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control field 20250625122509.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-6020
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245 10 - TITLE STATEMENT
Title Cyclin A/Cdk2 complexes regulate activation of Cdk1 and Cdc25 phosphatases in human cells
490 0# - SERIES STATEMENT
Volume/sequential designation Oncogene, 23(19), p.3361-3367, 2004
520 3# - SUMMARY, ETC.
Summary, etc. Mitotic entry, a critical decision point for maintaining genetic stability, is governed by the cyclin B/Cyclin dependent kinase 1 (Cdc2)complex. In Xenopus oocytes and early embryos, accumulation of cyclin B activates Cdk1, which then phosphorylates and activates the positive regulator Cdc25 in an autocatalytic feedback loop. However, cyclin B levels do not increase as some human cells approach mitosis, and the key factors regulating Cdk1 activation in human cells are unknown. We report here that reducing cyclin A expression by RNA interference (RNAi)in primary human fibroblasts inhibited activation of Cdc25B and Cdc25C and dephosphorylation of Cdk1 on tyrosine (tyr)15. These results were reproduced in U2-OS cells by inducing the expression of a dominant-negative (dn)mutant of Cdk2, the principal cyclin A binding partner. Cdk2-dn induction could inhibit Cdc25B activity and foster Cdk1 tyr phosphorylation within the S phase, temporally dissociating these events from Cdk1 activation at mitosis. In contrast, reducing Cdk1 expression delayed mitotic entry without markedly impairing Cdc25B or Cdc25C activity. These results suggest that cyclin A/Cdk2 complexes are key regulators of Cdc25 and Cdk1 activation in human cells. This pathway appears to be commonly deregulated in cancer.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CELL CYCLE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CDK1
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CDK2
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CDC25B
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CDC25C
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element G2
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Mitra, J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Enders, G.H.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1aRd1CLL_7sQEfP_-UXirdhk6oMBJx4el/view?usp=drivesdk">https://drive.google.com/file/d/1aRd1CLL_7sQEfP_-UXirdhk6oMBJx4el/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-6020 25.06.2025 25.06.2025 Documentos solicitados