Exploring Infection of Wheat and Carbohydrate Metabolism in Mycosphaerella graminicola Transformants with Differentially Regulated Green Fluorescent Protein Expression. (Record no. 40377)
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| fixed length control field | 02255nam a2200205Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625122509.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-6032 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Exploring Infection of Wheat and Carbohydrate Metabolism in Mycosphaerella graminicola Transformants with Differentially Regulated Green Fluorescent Protein Expression. |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Molecular Plant-Microbe Interactions, 14(2), p.156-163, 2001 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | A Mycosphaerella graminicola strain transformed with the green fluorescent protein (GFP)downstream of either a carbon source-repressed promoter or a constitutive promoter was used to investigate in situ carbohydrate uptake during penetration of the fungus in wheat leaves. The promoter region of the acu-3 gene from Neurospora crassa encoding isocitrate lyase was used as a carbon source-repressed promoter. The promoter region of the Aspergillus nidulans gpdA gene encoding glyceraldehyde-3-phosphate dehydrogenase was used as a constitutive promoter. Fluorometric measurement of GFP gene expression in liquid cultures of acu-3-regulated transformants indicated that the N. crassa acu-3 promoter functions in M. graminicola as it does in N. crassa, i.e., acetate induced and carbon source repressed. Glucose, fructose, and saccharose triggered the repression, whereas mannitol, xylose, and cell wall polysaccharides did not. Monitoring the GFP level during fungal infection of wheat leaves revealed that acu-3 promoter repression occurred after penetration until sporulation, when newly differentiated pycnidiospores fluoresced. The use of GFP transformants also allowed clear visualization of M. graminicola pathogenesis. No appressoria were formed, but penetration at cell junctions was observed. These results give new insight into the biotrophic status of M. graminicola. Additional keywords: plant-pathogenic fungus, septoria blotch, Septoria tritici, UB7. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Rohel, E.A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Payne, A.C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Fraaije, B.A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hollomon, D.W. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1VH2wQOHm_N81uEky65htv5EBwRLwnIFA/view?usp=drivesdk">https://drive.google.com/file/d/1VH2wQOHm_N81uEky65htv5EBwRLwnIFA/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-6032 | 25.06.2025 | 25.06.2025 | Documentos solicitados |