Electrophoretic Karyotype of the Obligate Biotrophic Parasite Plasmodiophora brassicae Wor. (Record no. 40394)

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fixed length control field 01722nam a2200253Ia 4500
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control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625122510.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-6049
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245 10 - TITLE STATEMENT
Title Electrophoretic Karyotype of the Obligate Biotrophic Parasite Plasmodiophora brassicae Wor.
490 0# - SERIES STATEMENT
Volume/sequential designation Journal of PhytoPathology, 149(6), p.313-318, 2001
520 3# - SUMMARY, ETC.
Summary, etc. Classical genetic analysis is not possible with the protist Plasmodiophora brassicae due to the intracellular life of this obligate biotrophic parasite. An electrophoretic karyotype has been obtained using contour-clamped homogeneous electric field gel electrophoresis to facilitate gene mapping of P. brassicae. Using two different separation conditions 16 chromosomal bands of P. brassicae were distinguished ranging in approximate size from 2.2 Mb to 680 kb. According to this determination of chromosome number and size, the total genome size of P. brassicae was estimated to be 20.3 Mb. The chromosomal bands were further designated by their hybridization pattern with repetitive elements of P. brassicae. The repetitive element H4 (1800 bp)hybridized with 14 chromosomal bands, but the sequence of H4 showed no homology to known centromere or telomere structures and revealed no repetitive motifs.
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Topical term or geographic name entry element CLUBROOT
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CHROMOSOME NUMBER
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Graf, H.
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Personal name Sokolowski, F.
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Personal name Klewer, A.
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Personal name Diederichsen E.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Luerßen, H.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Siemens J.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1ePqRnwLbP6XfPh5R7_n1Tk8lgA3xfHvH/view?usp=drivesdk">https://drive.google.com/file/d/1ePqRnwLbP6XfPh5R7_n1Tk8lgA3xfHvH/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-6049 25.06.2025 25.06.2025 Documentos solicitados