Revised Protocols for High Efficient Transformation and Regeneration of Somatic Embryos of Papaya (Carica papaya L.) (Record no. 40424)

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control field 20250625122510.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-6079
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245 10 - TITLE STATEMENT
Title Revised Protocols for High Efficient Transformation and Regeneration of Somatic Embryos of Papaya (Carica papaya L.)
490 0# - SERIES STATEMENT
Volume/sequential designation Acta Horticulturae, 740, p.147-152, 2007
520 3# - SUMMARY, ETC.
Summary, etc. Efforts to improve plant tissue culture and transformation is necessary for the production of transgenic papaya resistant to viral diseases. Induction of somatic embryos in liquid culture reduced the time for papaya genetic transformation. In this study, growth medium containing supplements of abscisic acid (ABA)and 2,4-dichlorophenoxyacetic acid (2,4-D)was optimised to control papaya explants proliferation and development into a proembryogenic mass suitable for somatic embryo induction. Our findings indicated that somatic embryos cultured in half-strength liquid MS medium containing 22.5 ìM 2,4-D and 2.5 ìM ABA yielded higher cell mass (dry-weight basis)than parallel treatments with other combinations of plant growth regulators. Subsequent sub-culture of the somatic embryos resulted in a population of somatic embryos at various stages of development. By repeated sieving of the embryogenic mass, through a stainless steel mesh with pore size of 250, 500 and 710 ìm, it was possible to separate the friable embryogenic calli and thus maintain synchronous suspension cultures. No significant difference was found in transient expression and regeneration of somatic embryos of different sizes after sieving. Rooting of papaya plantlets during acclimatization was the limiting factor for regeneration of papaya. Papaya plantlets rooted in husk infused media with an equal amount of half-strength MS medium showed an increase in the root length (453 cm), the number of root tips (20 tips)and survival rate (90
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PAPAYA
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SOMATIC EMBRYO
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TRANSFORMATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element REGENERATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ROOTING MEDIA
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Romyanon, K.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Boonthum, M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Attathom, S.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1voDyygkdn1nVjMWxVS_7AdsjTR4lPZHc/view?usp=drivesdk">https://drive.google.com/file/d/1voDyygkdn1nVjMWxVS_7AdsjTR4lPZHc/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-6079 25.06.2025 25.06.2025 Documentos solicitados