MARC details
| 000 -LEADER |
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| fixed length control field |
02270nam a2200361Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625122511.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-6104 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Phage lambda cDNA cloning vectors for subtractive hybridization, fusion-protein synthesis and Cre-loxP automatic plasmid subcloning |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Gene, 88(1), p.25-36, 1990 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
We describe the construction and use of two classes of cDNA cloning vectors. The first class comprises the EXLX(+)and EXLX(-)vectors that can be used for the expression in Escherichia coli of protein encoded by cDNA inserts is achived by the fusion of cDNA open reading frames to the T7 gene 10 promoter and protein-coding sequences. The second class, the SHLX vectors, allows the generation of large amounts of single-stranded DNA or synthetic cRNA that can be used in subtractive hybridization procedures. Both classes of vectors are designed to allow directional cDNA cloning with non-enzymatic protection of internal restriction sites. In addition, they are designed to facilitate conversion from phage to plasmid clones using a genetic method based on the bacteriophage P1 site-specific recombination system; we refer to this as automatic Cre-loxP plasmid subcloning. The phage arms, LOX, used in the construction of these vectors have unique restriction sites positioned between the two loxP sites. Insertion of specialized plasmid between these items sites will convert it into a phage cDNA cloning vector with automatic plasmid subcloning capability. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
RECOMBINANT DNA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
BACTERIOPHAGES |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
T7 |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
F1 |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
P1 |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
E. COLI RNA POLYMERASE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
PROMOTERS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
SITE-SPECIFIC RECOMBINATION |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Palazzoloa, M.J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Hamiltona, B.A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Ding, D. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Martinb, C.H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Meadc, D.A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Mierendorfd, R.C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Raghavana, K.V. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Raghavana, K.V. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Lipshhitz, H.D. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1aGtJnkxmGU8MEPF27OL-crdy3SlYfBQd/view?usp=drivesdk">https://drive.google.com/file/d/1aGtJnkxmGU8MEPF27OL-crdy3SlYfBQd/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
