Tagging and localization of a phospholipase D gene in Coprinellus congregatus by restriction enzyme-mediated integration and pulsed-field gel electrophoresis (Record no. 40453)

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control field 20250625122511.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-6108
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Title Tagging and localization of a phospholipase D gene in Coprinellus congregatus by restriction enzyme-mediated integration and pulsed-field gel electrophoresis
490 0# - SERIES STATEMENT
Volume/sequential designation We have identified a phospholipase D gene (pld)fragment from a transformant generated by restriction enzyme-mediated integration in Coprinellus congregatus, which is a mushroom-forming basidiomycete. A fragment of pld of this fungus has been cloned from a transformant by gene tagging. The transformation vector has been inserted into pld and this has resulted in a decreased enzyme activity of the transformant compared with the wild strain. C. congregatus has seven chromosomes, the range of its genome size is 1.6-4.7 Mb and pld is located at chromosome 4 where the transformation vector has been inserted., 225(2), p.285-289, 2003
520 3# - SUMMARY, ETC.
Summary, etc. We have identified a phospholipase D gene (pld)fragment from a transformant generated by restriction enzyme-mediated integration in Coprinellus congregatus, which is a mushroom-forming basidiomycete. A fragment of pld of this fungus has been cloned from a transformant by gene tagging. The transformation vector has been inserted into pld and this has resulted in a decreased enzyme activity of the transformant compared with the wild strain. C. congregatus has seven chromosomes, the range of its genome size is 1.6-4.7 Mb and pld is located at chromosome 4 where the transformation vector has been inserted.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PHOSPHOLIPASE D
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element GENE TAGGING
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PLASMID RESCUE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PULSED-FIELD GEL ELECTROPHORESIS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Leema, Y.E.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Rossb, I.K.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Choi, H.T.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1Rye-L45WD2vXkW0WYrJEsAgOBgomidb6/view?usp=drivesdk">https://drive.google.com/file/d/1Rye-L45WD2vXkW0WYrJEsAgOBgomidb6/view?usp=drivesdk</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-6108 25.06.2025 25.06.2025 Documentos solicitados