A user's guide to restriction enzyme-mediated integration in Dictyostelium (Record no. 40455)

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control field 20250625122511.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-6110
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Title A user's guide to restriction enzyme-mediated integration in Dictyostelium
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Volume/sequential designation Journal of Muscle Research and Cell Motility, 23 (7-8), p.597-604, 2002
520 3# - SUMMARY, ETC.
Summary, etc. Restriction enzyme-mediated integration (REMI)has been used to study a number of cellular and developmental processes in Dictyostelium discoideum. In this paper we review the basics of this powerful method of introducing random mutations in Dictyostelium. Here we discuss several mutation screens that have been devised and some of the genes that have been discovered through this approach to mutagenesis. Included in this discussion is how one goes about isolating a gene that has been disrupted by REMI, and how one confirms that this disruption is actually responsible for the observed phenotype. Finally, we describe how REMI can be used as an effective teaching tool in undergraduate cell biology laboratory courses.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Guerin, N.A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Larochelle, D.A.
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Uniform Resource Identifier <a href="https://drive.google.com/file/d/1wLB39I-UVrx22K02_m_z-yvaASueRt3O/view?usp=drivesdk">https://drive.google.com/file/d/1wLB39I-UVrx22K02_m_z-yvaASueRt3O/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-6110 25.06.2025 25.06.2025 Documentos solicitados