MARC details
| 000 -LEADER |
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| fixed length control field |
02516nam a2200301Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625122511.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-6111 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Identification of plant-regulated genes in Ustilago maydis by enhancer-trapping mutagenesis |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Molecular Genetics and Genomics, 270(4), p.303 - 314, 2003 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
To identify plant-induced genes in the maize pathogenic fungus Ustilago maydis we have developed a genetic screen that combines REMI (restriction enzyme mediated integration)mutagenesis with enhancer trapping using the gene for Green Fluorescent Protein (GFP)as vital reporter. Of 2350 insertion mutants isolated, three were shown to express GFP only after the fungus had come into contact with the host maize plant. One of the genes tagged was mfa1, which encodes the pheromone precursor, while the second gene, pig2 , codes for a product that showed similarity to protein disulfide isomerase. The third integration event had occurred in a locus which we designated the p -locus. This locus contains 11 genes in a 24-kb stretch. Of these, pig3 , 4 , 5 , 6 and 7 show a plant-regulated expression pattern, while the other genes found at the locus (designated npi)do not. Of the plant-regulated genes only two were found to be similar to database entries: the pig4 product is related to membrane transporters of the major facilitator family, while the pig6 protein shows similarity to multidrug transporters. Detailed expression studies revealed that the five plant-regulated genes at the p -locus differ in their expression profiles. Mutants deleted for each of them showed no apparent phenotype, while the npi1 gene appeared to be essential. A viable deletion encompassing the entire p -locus could be generated when npi1 function was provided ectopically. This deletion mutant also showed no obvious alteration in virulence. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
TAGGING MUTAGENESIS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
ENHANCER TRAP |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
RESTRICTION-ENZYME MEDIATED INTEGRATION (REMI) |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
PLANT-INDUCED GENE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
PATHOGENICITY |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Aichinger, C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Hansson, K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Eichhorn, H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Lessing, F. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Mannhaupt, G. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Mewes, W. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Kahmann, R. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1ueMv9wKr-tnJbpm6B-pCMlA9LL1iQtGK/view?usp=drivesdk">https://drive.google.com/file/d/1ueMv9wKr-tnJbpm6B-pCMlA9LL1iQtGK/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
