Efficacy of a Recombinant Chimeric Anti-hCG Antibody to Prevent Human Cytotrophoblasts Fusion and Block Progesterone Synthesis (Record no. 40482)

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control field 20250625124637.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-6137
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Title Efficacy of a Recombinant Chimeric Anti-hCG Antibody to Prevent Human Cytotrophoblasts Fusion and Block Progesterone Synthesis
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Volume/sequential designation American Journal of Reproductive Immunology, 51(5), p.358-363, 2004
520 3# - SUMMARY, ETC.
Summary, etc. Dhar R, Karmakar S, Sriraman R, Talwar GP, Das C. Efficacy of a recombinant chimeric anti-hCG antibody to prevent human cytotrophoblasts fusion and block progesterone synthesis. AJRI 2004; 51:358-363 © Blackwell Munksgaard, 2004 Problem: A recombinant chimeric antibody against hCG (cPIPP)has been engineered and expressed at high yield in plants. The purpose of this work was to enquire whether this antibody is competent to neutralize the bioactivity of hCG on human trophoblasts. Methods: Cytotrophoblast cells, isolated from term placentae were maintained in culture for 3 days in presence or absence of humanized chimeric anti-hCG antibodies. Progesterone secreted was quantitated by ELISA. Fusion and cyto-architecture of the cells was studied by light and electron microscopy. Modulation of E-cadherin was investigated using RT-PCR and immunocytochemistry. Results: Recombinant chimeric anti-hCG antibody blocked the synthesis of progesterone by trophoblasts. No fusion of cytotrophoblasts to form syncytium took place. E-cadherin, a vital cell adhesion molecule involved in cell-to-cell interaction did not show differentiation related decline in its expression in presence of the antibody. Conclusion: Recombinant chimeric anti-hCG antibody (cPIPP)was effective to neutralize hCG induced bioactivities in the human derived trophoblast cells.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element E-CADHERIN
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Topical term or geographic name entry element HCG
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Topical term or geographic name entry element TROPHOBLAST DIFFERENTIATION
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Personal name Dhar R.
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Personal name Karmakar S.
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Personal name Sriraman R.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Talwar G.P.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/104OkWtVw8lm7kkRqEcTgs9SKuKIwxTkS/view?usp=drivesdk">https://drive.google.com/file/d/104OkWtVw8lm7kkRqEcTgs9SKuKIwxTkS/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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