Application of Bacterial Artificial Chromosomes to the Generation of Contiguous Physical Maps: A Pilot Study of Human Ryanodine Receptor Gene (RYR1)Region (Record no. 40629)

MARC details
000 -LEADER
fixed length control field 01623nam a2200193Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625124640.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-6284
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 250602s9999 xx |||||s2 |||| ||und|d
245 10 - TITLE STATEMENT
Title Application of Bacterial Artificial Chromosomes to the Generation of Contiguous Physical Maps: A Pilot Study of Human Ryanodine Receptor Gene (RYR1)Region
490 0# - SERIES STATEMENT
Volume/sequential designation Analytical BioChemistry , 217(2), p.205-209, 1994
520 3# - SUMMARY, ETC.
Summary, etc. In order to increase the rate of generation of a contiguous human chromosome 19 physical map, we have investigated the advantages of using a bacterial artificial chromosome (BAC)library in comparison with other systems. This cloning system, recently described, can faithfully propagate DNA fragments greater than 300 kb in size. We have screened a total human genomic BAC library with a complex cosmid probe, specific for the ryanodine receptor gene (RYR1)located at 19q13.1. One 150-kb BAC was positive for the ryanodine receptor probe. The ryanodine receptor BAC hybridized to a 150-kb overlapping set of nine chromosome 19-specific cosmids at the 3' terminus of the RYR1 gene as well as two established cosmid contigs which can be linked to the same physical region. The hybridization of the BAC to a 150-kb set of chromosome 19 cosmids suggests that this BAC is nonchimeric instructure.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Rouquier S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Batzer M.A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Giorgi D.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1E2cQMcH0xgPpcnOz4F53hE5oAWjlIzqI/view?usp=drivesdk">https://drive.google.com/file/d/1E2cQMcH0xgPpcnOz4F53hE5oAWjlIzqI/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
Holdings
Lost status Source of classification or shelving scheme Damaged status Not for loan Collection Home library Current library Shelving location Date acquired Total checkouts Full call number Date last seen Price effective from Koha item type
  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-6284 25.06.2025 25.06.2025 Documentos solicitados