Effect of immobilization on pH and thermal stability of Aspergillus ficuum phytase (Record no. 40695)

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control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625124641.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-6351
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
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245 10 - TITLE STATEMENT
Title Effect of immobilization on pH and thermal stability of Aspergillus ficuum phytase
490 0# - SERIES STATEMENT
Volume/sequential designation Enzyme and Microbial Technology, 25, p.517-521, 1999
520 3# - SUMMARY, ETC.
Summary, etc. The phytase from Aspergillus ficuum catalyzes the hydrolysis of phytic acid into phosphoric acid and myo-inositol. The activity of this enzyme was determined by monitoring the rate of inositol production using high-performance liquid chromography methodology. The maximum activity of the enzyme was found to be approximately pH 5 and had a temperature optimum of 50°C. Under this condition, the kcat of 96 s-1 was obtained, and the corresponding Km for the catalysis of phytic acid was 2.34 mM. The optimum pH for the immobilized phytase was not much different from the intact enzyme. However, the optimum temperature was increased to 58°C, which is 8°C higher than that of free enzyme. Apparent Km for the immobilized enzyme was 3.28 mM, and only 34.6
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ASPERGILLUS FICUUM
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PHYTASE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MYO-INOSITOL
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Liu, B-L.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Jong, C-H.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Tzeng, Y.-M.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1h_3-zyJx5bJNlWCIO-fXNkJ0IZxCdfd2/view?usp=drivesdk">https://drive.google.com/file/d/1h_3-zyJx5bJNlWCIO-fXNkJ0IZxCdfd2/view?usp=drivesdk</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-6351 25.06.2025 25.06.2025 Documentos solicitados