REMI-induced mutants ofMycosphaerella zeae-maydislacking the polyketide PM-toxin are deficient in pathogenesis to corn (Record no. 40777)

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fixed length control field 01568nam a2200193Ia 4500
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control field MX-MdCICY
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control field 20250625124643.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-6433
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245 10 - TITLE STATEMENT
Title REMI-induced mutants ofMycosphaerella zeae-maydislacking the polyketide PM-toxin are deficient in pathogenesis to corn
490 0# - SERIES STATEMENT
Volume/sequential designation Physiological and Molecular Plant Pathology, 52(1), p.53-66, 1998
520 3# - SUMMARY, ETC.
Summary, etc. The polyketide PM-toxin, produced byMycosphaerella zeae-maydis, is specifically active against corn containing Texas male sterile (T)cytoplasm, as is the fungus itself. To determine if PM-toxin is required for disease development,Tox-mutants were generated using the restriction enzyme-mediated integration (REMI)strategy, which is designed to tag the mutations it creates. As a mutagenic procedure, REMI was highly efficient, yielding five stable PM-toxin-deficient mutants among 504 transformants recovered. AllTox-mutants lost the ability to cause disease on T-cytoplasm corn, establishing a role for PM-toxin in pathogenesis. Examination of genomic DNAs from theTox-mutants revealed one that carried the transformation vector at multiple insertion sites and two that had no apparent ORFs at single vector insertion sites; three sustained deletions.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Yun, S.-H.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Turgeon, B.G.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Yoder, O.C.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1RuHyuwPt8LtxPDX5v5xeHKm7k5eQeTyw/view?usp=drivesdk">https://drive.google.com/file/d/1RuHyuwPt8LtxPDX5v5xeHKm7k5eQeTyw/view?usp=drivesdk</a>
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