Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine (Record no. 40782)
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| fixed length control field | 01798nam a2200169Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625124643.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-6438 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Analytical BioChemistry, 106(1), p.207-212, 1980 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | The total glutathione content of biological samples is conveniently determined with an enzymatic recycling assay based on glutathione reductase ([1.], Anal. Biochem. 27, 502-522). In the original and several subsequent descriptions of this procedure, glutathione disulfide is selectively determined by assaying samples in which glutathione is masked by pretreatment with N-ethylmaleimide. Since N-ethylmaleimide is a potent inhibitor of glutathione reductase, it is necessary to remove excess reagent; the procedures used are laborious and contribute significantly to experimental error. It is reported here that 2-vinylpyridine is a much better reagent for the derivitization of glutathione. In contrast to N-ethylmaleimide, 2-vinylpyridine does not inhibit glutathione reductase significantly and therefore need not be removed from the sample solutions. 2-Vinylpyridine reacts with glutathione at slightly acidic pH values where spontaneous formation of glutathione disulfide is minimal. It is demonstrated that the total glutathione concentration in mouse plasma is substantially higher than generally reported and that glutathione disulfide constitutes less than 30 |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Griffith, O.W. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1PGXDQrjL8V2pB9Vdk573qV_OPcEMflX9/view?usp=drivesdk">https://drive.google.com/file/d/1PGXDQrjL8V2pB9Vdk573qV_OPcEMflX9/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-6438 | 25.06.2025 | 25.06.2025 | Documentos solicitados |