A redox-dependent, G-protein-coupled phospholipase A of the plasma membrane is involved in the elicitation of alkaloid biosynthesis in Eschscholtzia californica (Record no. 40807)
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| fixed length control field | 02177nam a2200229Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625124643.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-6464 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | A redox-dependent, G-protein-coupled phospholipase A of the plasma membrane is involved in the elicitation of alkaloid biosynthesis in Eschscholtzia californica |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Biochimica et Biophysica Acta (BBA)/Molecular Cell Research, 1448(3), p.390-402, 1999 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | In cultured cells of Californian poppy formation of benzophenanthridine alkaloids can be triggered by a yeast elicitor preparation independently of the hypersensitive reaction. A plasma membrane (PM)bound phospholipase A (PLA)is likely to play a role in the signalling process: PLA activity was detectable in individual cells, cell suspensions and PM vesicles with the fluorogenic phospholipid bis-BODIPY FL C11-PC and was sensitive to known inhibitors of PLA2. In microscopic assays, enzyme activity increased after elicitor contact of cells that were pretreated with non-saturating concentrations of PLA2 inhibitors. In PM vesicles a PLA2-like protein as well as Ga- and Gß-proteins were detected immunologically. Anti-Ga or anti-Gß antisera or mastoparan stimulated PLA activity thus indicating a G-protein-controlled enzyme. Elicitation of alkaloid production was sensitive to aristolochic acid and enhanced by PLA2 products such as lysophosphatidylcholine and linolenic acid. Pretreatment of the cells with the artificial electron acceptors hexabromoiridate(V)or ferricyanide(III)reversibly abolished the effect of subsequent elicitation and reduced the activity of PLA both in intact cells and in PM vesicles. It appears, therefore, that PLA2 is a point of interference of redox control with the signal path. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Roos, W. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Dordschbal, B. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Steighardt, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hieke, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Weiss, D. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Saalbach, G. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1mlDi_CJFiQkbOD4b8fD7sY4B0uNxQWNJ/view?usp=drivesdk">https://drive.google.com/file/d/1mlDi_CJFiQkbOD4b8fD7sY4B0uNxQWNJ/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-6464 | 25.06.2025 | 25.06.2025 | Documentos solicitados |