A Large-Insert (130 kbp)Bacterial Artificial Chromosome Library of the Rice Blast FungusMagnaporthe grisea:Genome Analysis, Contig Assembly, and Gene Cloning (Record no. 40809)
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| fixed length control field | 01907nam a2200217Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625124643.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-6466 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | A Large-Insert (130 kbp)Bacterial Artificial Chromosome Library of the Rice Blast FungusMagnaporthe grisea:Genome Analysis, Contig Assembly, and Gene Cloning |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Fungal Genetics and Biology, 21(3), p.337-347, 1997 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Magnaporthe grisea(Hebert)Barr causes rice blast, one of the most devastating diseases of rice (Oryza sativa)worldwide. This fungus is an ideal organism for studying a number of aspects of plant-pathogen interactions, including infection-related morphogenesis, avirulence, and pathogen evolution. To facilitateM. griseagenome analysis, physical mapping, and positional cloning, we have constructed a bacterial artificial chromosome (BAC)library from the rice infecting strain 70-15. A new method was developed for separation of partially digested large-molecular-weight DNA fragments that facilitated library construction with large inserts. The library contains 9216 clones, with an average insert size of 130 kbp (>25 genome equivalents)stored in 384-well microtiter plates that can be double spotted robotically on to a single nylon membrane. Several unlinked single-copy DNA probes were used to screen 4608 clones in the library and an average of 13 (minimum of 6)overlapping BAC clones was found in each case. Hybridization of total genomic DNA to the library and analysis of individual clones indicated that ~26 |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zhu, H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Choi, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Johnston, A.K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Wing, R.A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Dean, R.A. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1_irRuNWngWeTMJ2wrEzPtSz6Sike1aQD/view?usp=drivesdk">https://drive.google.com/file/d/1_irRuNWngWeTMJ2wrEzPtSz6Sike1aQD/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-6466 | 25.06.2025 | 25.06.2025 | Documentos solicitados |