Geminivirus Resistance in Transgenic Tobacco Expressing Mutated BC1 Protein. (Record no. 40820)
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| fixed length control field | 01963nam a2200217Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625124643.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-6477 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Geminivirus Resistance in Transgenic Tobacco Expressing Mutated BC1 Protein. |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Molecular Plant-Microbe Interactions, 10(7), p.617-623, 1997 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Tobacco explants were transformed by Agrobacterium-mediated transformation with sense and antisense constructs of the movement protein genes (BC1 and BV1)of tomato mottle geminivirus (TMoV). Transgenic plants were tested for virus resistance either by exposure to viruliferous whiteflies carrying TMoV or cabbage leaf curl geminivirus (CabLCV)for a 72-h inoculation period or by continuous exposure to TMoV during the life of the plants. The transgenic lines were scored for disease symptoms, and virus replication and distribution were determined by enzyme-linked immunosorbent assay and dot blot hybridizations. Transgenic plants which expressed a mutated form (identified in a previous study)of the BC1 gene showed TMoV and CabLCV resistance. Three resistant phenotypes were observed: a delay in symptom development, a recovery from early symptoms, and an absence of virus symptoms at all stages. Geminivirus was detected in inoculated leaves but was not readily detected in leaves beyond the inoculation sites in the highly resistant plants. The geminivirus resistance conferred by expression of the spontaneously mutated BC1 gene (detected after transformation)in transgenic tobacco may involve transdominant negative interference. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Duan, Y.P. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Powell, C.A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Webb, S.E. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Purcifull, D.E. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hiebert, E. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/16sv8NtEAnlCloiYjN-fcFNZq6LzTV9Z-/view?usp=drivesdk">https://drive.google.com/file/d/16sv8NtEAnlCloiYjN-fcFNZq6LzTV9Z-/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-6477 | 25.06.2025 | 25.06.2025 | Documentos solicitados |