Mutational analysis of loxP sites for efficient Cre-mediated insertion into genomic DNA. (Record no. 40868)

MARC details
000 -LEADER
fixed length control field 01656nam a2200193Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625124644.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-6526
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 250602s9999 xx |||||s2 |||| ||und|d
245 10 - TITLE STATEMENT
Title Mutational analysis of loxP sites for efficient Cre-mediated insertion into genomic DNA.
490 0# - SERIES STATEMENT
Volume/sequential designation Genesis, 36(3), p.162-7, 2003
520 3# - SUMMARY, ETC.
Summary, etc. The Cre/loxP system has been used in transgenic models primarily to excise DNA flanked by loxP sites for gene deletion. However, the insertion reaction is more difficult to control since the excision event is kinetically favored. Mutant loxP sites favoring integration were identified using a novel, bacterial screening system. Utilizing lambda integrase, mutant loxP sites were placed at the E. coli attB site and the excision-insertion ratios of incoming DNA plasmids carrying a second, complementary mutant loxP site were determined. Comparison of 50 mutant loxP sites combinations to the native loxP site revealed that mutations to the inner 6 bp of the Cre binding domain severely inhibited recombination, while those in the outer 8 bps were more tolerated. The most efficient loxP combinations resulted in 1421-fold and 1529-fold increases in relative integration rates over wild-type loxP sites. These loxP mutants could be exploited for site-directed "tag and insert" recombination experiments
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Thomson, JG
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Rucker, E.B.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Piedrahita, J.A.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1n8KLnoCXOUJRg62HnB7jtZwFLQxVblW6/view?usp=drivesdk">https://drive.google.com/file/d/1n8KLnoCXOUJRg62HnB7jtZwFLQxVblW6/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
Holdings
Lost status Source of classification or shelving scheme Damaged status Not for loan Collection Home library Current library Shelving location Date acquired Total checkouts Full call number Date last seen Price effective from Koha item type
  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-6526 25.06.2025 25.06.2025 Documentos solicitados