Cloned single- and double-stranded DNA copies of potato spindle tuber viroid (PSTV)RNA and co-inoculated subgenomic DNA fragments are infectious (Record no. 40876)
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| fixed length control field | 01856nam a2200181Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625124644.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-6535 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Cloned single- and double-stranded DNA copies of potato spindle tuber viroid (PSTV)RNA and co-inoculated subgenomic DNA fragments are infectious |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | The EMBO Journal, 13, p.3055-3062, 1984 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | A set of monomeric and oligomeric potato spindle tuber viroid (PSTV)specific DNA forms representing complete DNA copies of the circular PSTV RNA genome were constructed and cloned in plasmid pBR322 and bacteriophage M13. Both single- and double-stranded PSTV DNAs are capable of initiating viroid replication in mechanically inoculated tomato plants where it normally proceeds via the RNA-RNA pathway without DNA being involved. All dimeric and higher multimeric forms were infectious irrespective of their polarity in the case of single- stranded DNA and regardless of their orientation in the vector DNA in the case of double-stranded DNA. The vector-inserted monomeric PSTV DNA units were also found to be infectious but of low specific infectivity which was increased when these monomers had been excised. Even two subgenomic DNA fragments, representing together the 359 nucleotides of the PSTV RNA genome, initiated the synthesis of viroid RNA progeny when co-inoculated although each fragment by itself is non-infectious. These results are discussed with respect to the infectivity previously observed with certain cloned DNAs of conventional RNA and DNA viruses. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Tabler, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sanger, H.L. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1ABRWmVNgm4_2ZcQiYEup5tKaQmKgMfdu/view?usp=drivesdk">https://drive.google.com/file/d/1ABRWmVNgm4_2ZcQiYEup5tKaQmKgMfdu/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-6535 | 25.06.2025 | 25.06.2025 | Documentos solicitados |