The stem-loop region of the tobacco psbA 5'UTR is an important determinant of mRNA stability and translation efficiency (Record no. 40880)

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control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625124644.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-6539
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245 10 - TITLE STATEMENT
Title The stem-loop region of the tobacco psbA 5'UTR is an important determinant of mRNA stability and translation efficiency
490 0# - SERIES STATEMENT
Volume/sequential designation Molecular Genetics and Genomics , 269(3), p.340-349, 2003
520 3# - SUMMARY, ETC.
Summary, etc. Regulation of chloroplast gene expression involves networked and concerted interactions of nucleus-encoded factors with their target sites on untranslated regions (UTRs)of chloroplast transcripts. So far, only a few cis-acting elements within such 5'UTR sequences have been identified as functional determinants of mRNA stability and efficient translation in Chlamydomonasin vivo. In this study, we have used chloroplast transformation and site-directed mutagenesis to analyse the functions of the 5'UTRs of tobacco psbAand rbcLfused to the coding region of the reporter gene uidA. Various mutant versions of the psbAleader, as well as rbcL/psbAhybrid leader elements, were investigated. Our results showed a 1.5- to 3-fold decrease in uidAmRNA levels and a 1.5- to 6-fold reduction in uidAtranslation efficiency in all psbA5'UTR stem-loop mutants generated by sequence deletions and base alterations. This indicates that the correct primary sequence and secondary structure of the psbA5'UTR stem-loop are required for mRNA stabilisation and translation. The 5'-terminal segment of the rbcL5'UTR did not enhance the stability or translational activity of chimeric uidAmRNA under the standard light-dark regime of 16 h light and 8 h dark. Stabilising effects were, however, observed when the cells were kept continuously in the dark. Possible reasons for the influence of the 5'UTR of the tobacco psbA on mRNA stability and translation efficiency are discussed.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TOBACCO
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element 5'UTR
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MRNA STABILITY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TRANSLATION EFFICIENCY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CHLOROPLAST TRANSFORMATION
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Zou, Z.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Eibl, C.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Koop, H.U.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1wLirKdgsPzkM1LQQW3AeSfN2_LoJRwHq/view?usp=drivesdk">https://drive.google.com/file/d/1wLirKdgsPzkM1LQQW3AeSfN2_LoJRwHq/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-6539 25.06.2025 25.06.2025 Documentos solicitados