Solanum nigrum: A model ecological expression system and its tools (Record no. 41070)

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fixed length control field 02897nam a2200313Ia 4500
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control field MX-MdCICY
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control field 20250625124648.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-6731
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Title Solanum nigrum: A model ecological expression system and its tools
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Volume/sequential designation Molecular Ecology, 13, p.981-995, 2004
520 3# - SUMMARY, ETC.
Summary, etc. Plants respond to environmental stresses through a series of complicated phenotypic responses, which can be understood only with field studies because other organisms must be recruited for their function. If ecologists are to fully participate in the genomics revolution and if molecular biologists are to understand adaptive phenotypic responses, native plant ecological expression systems that offer both molecular tools and interesting natural histories are needed. Here, we present Solanum nigrum L., a Solanaceous relative of potato and tomato for which many genomic tools are being developed, as a model plant ecological expression system. To facilitate manipulative ecological studies with S. nigrum , we describe: (i)an Agrobacterium -based transformation system and illustrate its utility with an example of the antisense expression of RuBPCase , as verified by Southern gel blot analysis and realtime quantitative PCR; (ii)a 789-oligonucleotide microarray and illustrate its utility with hybridizations of herbivore-elicited plants, and verify responses with RNA gel blot analysis and real-time quantitative PCR; (iii)analyses of secondary metabolites that function as direct (proteinase inhibitor activity)and indirect (herbivore-induced volatile organic compounds)defences; and (iv)growth and fitness-estimates for plants grown under field conditions. Using these tools, we demonstrate that attack from flea beetles elicits: (i)a large transcriptional change consistent with elicitation of both jasmonate and salicylate signalling; and (ii)increases in proteinase inhibitor transcripts and activity, and volatile organic compound release. Both flea beetle attack and jasmonate elicitation increased proteinase inhibitors and jasmonate elicitation decreased fitness in field-grown plants. Hence, proteinase inhibitors and jasmonate-signalling are targets for manipulative studies.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element AGROBACTERIUM-MEDIATED TRANSFORMATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element HERBIVORE-INDUCED VOLATILE PRODUCTION
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Topical term or geographic name entry element INDIRECT AND DIRECT DEFENCES
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element OLIGONUCLEOTIDE MICROARRAY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PLANT FITNESS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PROTEINASE INHIBITOR
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Schmidt, D.D.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kessler, A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kessler, D.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Schmidt, S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Lim, M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Gase, K.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Baldwin, I.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1obz8sbTw6rhJ04tyM3Xk4eKL3_9rISBm/view?usp=drivesdk">https://drive.google.com/file/d/1obz8sbTw6rhJ04tyM3Xk4eKL3_9rISBm/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-6731 25.06.2025 25.06.2025 Documentos solicitados