Protoplast preparation and transient transformation of Rhizoctonia solani (Record no. 41095)

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control field MX-MdCICY
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control field 20250625124648.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-6756
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Title Protoplast preparation and transient transformation of Rhizoctonia solani
490 0# - SERIES STATEMENT
Volume/sequential designation Mycological Research, 105, p.1295-1303, 2001
520 3# - SUMMARY, ETC.
Summary, etc. Optimization of protoplast release (202×104 ml[minus sign]1)from a strain of Rhizoctonia solani anastomosis group three was achieved by treating 3 d old mycelia from broth cultures with Novozyme 234 for 3 h at 30 °C. The only other treatment, of many tested, that yielded any protoplasts (15·6×104 ml[minus sign]1)was commercial lysing enzymes from Trichoderma harzianum. Various combinations of commercial enzyme preparations, variations of osmotic stabilisers, and mycelial age, were ineffective in raising the protoplast yield. Regeneration of colonies from protoplasts was maximal (83-87
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Robinson, H.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Deacon, J.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1KnFioZnIOYVk6wIUcDkBzMPy3xnOFI0G/view?usp=drivesdk">https://drive.google.com/file/d/1KnFioZnIOYVk6wIUcDkBzMPy3xnOFI0G/view?usp=drivesdk</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-6756 25.06.2025 25.06.2025 Documentos solicitados