DSC studies on bovine serum albumin denaturation Effects of ionic strength and SDS concentration (Record no. 41181)

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control field 20250625124650.0
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Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-6843
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245 10 - TITLE STATEMENT
Title DSC studies on bovine serum albumin denaturation Effects of ionic strength and SDS concentration
490 0# - SERIES STATEMENT
Volume/sequential designation International Journal of Biological Macromolecules , 20(3), p.193-204, 1997
520 3# - SUMMARY, ETC.
Summary, etc. This work analyzed the thermal denaturation process of defatted bovine serum albumin (BSA). DSC measurements were performed on changing the pH, the ionic strength and the sodium dodecyl sulfate (SDS)concentration. These data have been compared with those previously obtained by us and other authors. The purpose of these measurements was to study the correlation between the three-dimensional organization of BSA native protein structure and its thermodynamic stability and to clarify the non-covalent interactions between the globular proteins and amphipathic molecules. These measurements have shown that the thermal denaturation is always irreversible regardless of pH, ionic strength and SDS concentration. The nature of the irreversible process superimposed on the protein unfolding is discussed. The strong stabilizing effect of NaC1 on the BSA native structure has been found for the range 0-1.0 M. It is worth noting that the calorimetric curves, confined to the pH region studied, could not be represented by a two-state transition model; they were deconvoluted as the sum of two independent two-state transitions. These transitions were correlated to the domain structure of BSA. Sodium dodecyl sulfate has a net stabilizing effect up to a molar ratio of 10:1 (ligand to protein). In this range of concentrations the presence of SDS causes a biphasic profile of excess heat capacity. A simple thermodynamic model was developed in attempt to reproduce the experimental DSC profiles and collect information regarding the binding equilibrium of SDS.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element BOVINE SERUM ALBUMIN
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PROTEIN DENATURATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SODIUM DODECYL SULFATE BINDING
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Giancola, C.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name De Sena, C.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Fessasa, D.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Graziano, G.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1tmOF4F6yplqfpbHqWnWGePyR2PVp9i-6/view?usp=drivesdk">https://drive.google.com/file/d/1tmOF4F6yplqfpbHqWnWGePyR2PVp9i-6/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-6843 25.06.2025 25.06.2025 Documentos solicitados