Purification and characterization of S-adenosyl-L-methionine :norcoclaurine 6-0-methyltransferase from cultured Coptis japonica cells (Record no. 41207)
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| fixed length control field | 01837nam a2200217Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625124650.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-6869 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Purification and characterization of S-adenosyl-L-methionine :norcoclaurine 6-0-methyltransferase from cultured Coptis japonica cells |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | European Journal of BioChemistry, 225, p.125-131, 1994 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | S-adenosyl-L-methionine:norcoclaurine 6-0-methyltransferase (norcoclaurine 6-0-methyltransferase), which catalyzes the transfer of the S-methyl group of S-adenosyl-L-methionine to the 6-hydroxyl group of 2,3,4-tetrahydro-l-[(4-hydroxyphenyl)methyl]-6,7-isoquinolinediol (norcoclaurine), was purified from cultured Coptisjuponica cells and its enzymic properties were characterized. Purified norcoclaurine 6-0-methyltransferase had apparent PI 4.7, a native molecular mass of 95 kDa (determined by gel filtration)and subunit molecular mass of 40 kDa (SDSFAGE). The enzyme did not require a divalent cation for activity, and the addition of Fez+, Cuz+, Co2+, Zn2+, Mn '+, or Ni2' at 5 mh4 severely inhibited enzyme activity. Neither p-chloromercuribenzoate, Nmethylmaleimide nor iodoacetamide inhibited enzyme activity at 1 mM. 5,6-Dihydro-9,10-dimethoxybenzo[ g]-l,3-benzodioxolo[5,6-u]quinolizinium(b erberine, the end-product of the biosynthetic pathway in which norcoclaurine 6-0-methyltransferase catalyzes an intermediate step)also inhibited the activity by 50 |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sato, F. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Tsujita, T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Katagiri, Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Yoshida, Sh. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Yamada, Y. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1o5nqVTp_Bq3xFK9UhWe0-Wl_L4_UaM00/view?usp=drivesdk">https://drive.google.com/file/d/1o5nqVTp_Bq3xFK9UhWe0-Wl_L4_UaM00/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-6869 | 25.06.2025 | 25.06.2025 | Documentos solicitados |