Confocal Direct Imaging Raman Microscope: Design and Applications in Biology (Record no. 41258)
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| 000 -LEADER | |
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| fixed length control field | 01983nam a2200277Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625124651.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-6921 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Confocal Direct Imaging Raman Microscope: Design and Applications in Biology |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Applied Spectroscopy, 52(3), p.348-355, 1998 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | A confocal direct imaging Raman microscope (CDIRM)based on two synchronized scanning mirrors, a monochromator, and two charge-coupled device (CCD)cameras has been developed. With this system it is possible to make both Raman spectra of a small measurement volume and images of a larger sample area in one speci® c Raman band. The spatial resolution of the system was determined for two limiting situations: a small sphere and a thin layer. The image of a 0.282 m m sphere appeared to have a full width at half-maximum (FWHM)of 1.2 m m in the axial and 0.37 m m in the lateral direction, whereas the image of a 275 nm layer showed an FWHM of 1.4 m m in the axial direction . Confocal Raman images were made of the DNA and protein distribution in polytene chromosomes with a relatively weak Raman signal [0.1 photons/(second ´pixel)]. Further, a three-dimensional Raman image of the drug distribution in a hthalocyanine-incubated ® xed cell is presented. These examples show that the CDIRM can be used to image samples with a weak Raman signal and that three-dimensional images of the distribution of speci® c molecules in a sample can be made |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | RAMAN |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | MICROSCOPY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | SPECTROSCOPY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | CONFOCAL |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | IMAGING |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sijtsema, N.M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Wouters, S.D. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | De Grauw, C.J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Otto, C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Greve, J. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1HxzYuPKZWKOIuFXQolb3bxflnZ2aH8-4/view?usp=drivesdk">https://drive.google.com/file/d/1HxzYuPKZWKOIuFXQolb3bxflnZ2aH8-4/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-6921 | 25.06.2025 | 25.06.2025 | Documentos solicitados |