A spectrophotometric assay of gamma-glutamylcysteine synthetase and glutathione synthetase in crude extracts from tissues and cultured mammalian cells. (Record no. 41309)
[ view plain ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 01904nam a2200265Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625124652.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-6972 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | A spectrophotometric assay of gamma-glutamylcysteine synthetase and glutathione synthetase in crude extracts from tissues and cultured mammalian cells. |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Chemico-Biological Interactions, 140(1), p.49-65, 2002 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | An assay of gamma-glutamylcysteine synthetase (gamma-GCS)and glutathione synthetase (GS)in crude extracts of cultured cells and tissues is described. It represents a novel combination of known methods, and is based on the formation of glutathione (GSH)from cysteine, glutamate and glycine in the presence of rat kidney GS for the assay of gamma-GCS, or from gamma-glutamylcysteine and glycine for the assay of GS. GSH is then quantified by the Tietze recycling method. Assay mixtures contain the gamma-glutamyl transpeptidase (GGT)inhibitor acivicin in order to prevent the degradation of gamma-glutamylcysteine and of the accumulating GSH, and dithiothreitol in order to prevent the oxidation of cysteine and gamma-glutamylcysteine. gamma-GCS and GS levels determined by this method are comparable to those determined by others. The method is suitable for the rapid determination of gamma-GCS GS in GGT-containing tissues and for the studies of induction of gamma-GCS and GS in tissue cultures. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Volohonsky, G. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Tuby, C.N. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Porat, N. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Wellman-Rousseau, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Visvikis, A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Leroy, P. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Rashi, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Rashi, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Stark, A.A. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1SYrWQWxVj5Vw1rY8qhvDkA2z9yCyWmQo/view?usp=drivesdk">https://drive.google.com/file/d/1SYrWQWxVj5Vw1rY8qhvDkA2z9yCyWmQo/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-6972 | 25.06.2025 | 25.06.2025 | Documentos solicitados |