Mutan produced in potato amyloplasts adheres to starch granules (Record no. 41438)

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fixed length control field 02011nam a2200217Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625124655.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-7106
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 250602s9999 xx |||||s2 |||| ||und|d
245 10 - TITLE STATEMENT
Title Mutan produced in potato amyloplasts adheres to starch granules
490 0# - SERIES STATEMENT
Volume/sequential designation Plant Biotechnology Journal, 3(3), p.341, 2005
520 3# - SUMMARY, ETC.
Summary, etc. Production of water-insoluble mutan polymers in Kardal potato tubers was investigated after expression of a full-length (Gtf I)and a truncated mutansucrase gene referred to as GtfICAT ( Gtf I without glucan-binding domain)from Streptococcus downei.Subsequent effects on starch biosynthesis at the molecular and biochemical levels were studied. Expression of the GtfICAT gene resulted in the adhesion of mutan material on starch granules, which stained red with erythrosine, and which was hydrolysed by xo-mutanase. In addition, GtfICAT -expressing plants exhibited a severely altered tuber phenotype and starch granule morphology in comparison to those expressing the full-length Gtf I gene. In spite of that, no structural changes at the starch level were observed. Expression levels of the sucroseregulated, AGPase and GBSSI genes were down-regulated in only the GTFICAT transformants, showing that GtfICAT expression interfered with the starch biosynthetic pathway. In accordance with the down-regulated AGPase gene, a lower starch content was observed in the GTFICAT transformants. Finally, the rheological properties of the GTFICAT starches were modified; they showed a higher retrogradation during cooling of the starch paste.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ADHESIVENESS, GLUCANBINDING DOMAIN, GLUCANSUCRASE, MUTAN, TRANSGENIC POTATO, TRUNCATION.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kok-Jacon, J.A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Vincken, J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Suurs, L.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Visser, R.G.F.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/16fODWaOjYy2gNJ1w6skmVWKAa_A8XYOf/view?usp=drivesdk">https://drive.google.com/file/d/16fODWaOjYy2gNJ1w6skmVWKAa_A8XYOf/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-7106 25.06.2025 25.06.2025 Documentos solicitados