Distribution of Phytoplasmas in Infected Plants as Revealed by Real-Time PCR and Bioimaging (Record no. 41445)

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control field MX-MdCICY
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control field 20250625124655.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-7114
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Title Distribution of Phytoplasmas in Infected Plants as Revealed by Real-Time PCR and Bioimaging
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Volume/sequential designation Molecular Plant-Microbe Interactions, 17, p.1175-1184, 2004
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Summary, etc. Phytoplasmas are cell wall-less bacteria inhabiting the phloem and utilizing it for their spread. Infected plants often show changes in growth pattern and a reduced crop yield. A quantitative real-time polymerase chain reaction (Q-PCR)assay and a bioimaging method were developed to quantify and localize phytoplasmas in situ. According to the Q-PCR assay, phytoplasmas accumulated disproportionately in source leaves of Euphorbia pulcherrima and, to a lesser extent, in petioles of source leaves and in stems. However, phytoplasma accumulation was small or nondetectable in sink organs (roots and sink leaves). For bioimaging, infected plant tissue was stained with vital fluorescence dyes and examined using confocal laser scanning microscopy. With a DNA-sensitive dye, the pathogens were detected exclusively in the phloem, where they formed dense masses in sieve tubes of Catharanthus roseus. Sieve tubes were identified by counterstaining with aniline blue for callose and multiphoton excitation. With a potentiometric dye, not all DNA-positive material was stained, suggesting that the dye stained metabolically active phytoplasmas only. Some highly infected sieve tubes contained phytoplasmas that were either inactive or dead upon staining
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Personal name Meyn Christensen, N.
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Personal name Nicolaisen, M.
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Personal name Hansen, M.
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Personal name Schulz, A.
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Uniform Resource Identifier <a href="https://drive.google.com/file/d/1X6PaDmYomvpHdE_7UfiIL-vqEUT6pnet/view?usp=drivesdk">https://drive.google.com/file/d/1X6PaDmYomvpHdE_7UfiIL-vqEUT6pnet/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-7114 25.06.2025 25.06.2025 Documentos solicitados