Polymorphism of PCR-based markers targeting exons, introns, promoter regions, and SSRs in maize and introns and repeat sequences in oat1 (Record no. 41452)

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control field MX-MdCICY
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control field 20250625124655.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-7121
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Title Polymorphism of PCR-based markers targeting exons, introns, promoter regions, and SSRs in maize and introns and repeat sequences in oat1
490 0# - SERIES STATEMENT
Volume/sequential designation Genome, 44(6), p.1065-1076, 2001
520 3# - SUMMARY, ETC.
Summary, etc. Sequence databases could be efficiently exploited for development of DNA markers if it were known which gene regions reveal the most polymorphism when amplified by PCR. We developed PCR primer pairs that target specific regions of previously sequenced genes from Avena and Zea species. Primers were targeted to amplify 40 introns, 24 exons, and 23 promoter regions within 54 maize genes. We surveyed 48 maize inbred lines (previously assayed for simple-sequence repeat (SSR)polymorphism)for implification-product polymorphism. We also developed primers to target 14 SSRs and 12 introns within 18 Avena genes, and surveyed 22 hexaploid oat cultivars and 2 diploid Avena species for amplification-product polymorphism. In maize, 67
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Holland, J.B.
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Personal name Helland, S.J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Sharopova, N.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Rhyne, D.C.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1xGkDAcDsdWYhEAohEXAwU6UYkzOqKEhg/view?usp=drivesdk">https://drive.google.com/file/d/1xGkDAcDsdWYhEAohEXAwU6UYkzOqKEhg/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-7121 25.06.2025 25.06.2025 Documentos solicitados