Genotyping single nucleotide polymorphisms in barley by tetra-primer ARMS-PCR (Record no. 41453)

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control field 20250625124655.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-7122
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Title Genotyping single nucleotide polymorphisms in barley by tetra-primer ARMS-PCR
490 0# - SERIES STATEMENT
Volume/sequential designation Genome, 47(2), p.414-420, 2004
520 3# - SUMMARY, ETC.
Summary, etc. Single nucleotide polymorphisms (SNPs)are the most abundant form of DNA polymorphism. These polymorphisms can be used in plants as simple genetic markers for many breeding applications, for population studies, and for germplasm fingerprinting. The great increase in the available DNA sequences in the databases has made it possible to identify SNPs by "database mining", and the single most important factor preventing their widespread use appears to be the genotyping cost. Many genotyping platforms rely on the use of sophisticated, automated equipment coupled to costly chemistry and detection systems. A simple and economical method involving a single PCR is reported here for barley SNP genotyping. Using the tetra-primer ARMS-PCR procedure, we have been able to assay unambiguously five SNPs in a set of 132 varieties of cultivated barley. The results show the reliability of this technique and its potential for use in low- to moderate-throughput situations; the association of agronomically important traits is discussed.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Chiapparino, E.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Lee, D.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Donini, P.
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Uniform Resource Identifier <a href="https://drive.google.com/file/d/1rER1VrDZ6sGl3vnaMmQ22SYVK5XUmfbF/view?usp=drivesdk">https://drive.google.com/file/d/1rER1VrDZ6sGl3vnaMmQ22SYVK5XUmfbF/view?usp=drivesdk</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-7122 25.06.2025 25.06.2025 Documentos solicitados