Cloning, characterization and overexpression of the phytase-encoding gene (phyA)of Aspergillus niger (Record no. 41458)
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| fixed length control field | 02007nam a2200289Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625124655.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-7127 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Cloning, characterization and overexpression of the phytase-encoding gene (phyA)of Aspergillus niger |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Gene, 127(1), p.87-94, 1993 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Phytase catalyzes the hydrolysis of phytate (myo-inositol hexakisphosphate)to myo-inositol and inorganic phosphate. A gene (phyA)of Aspergillus niger NRRL3 135 coding for extracellular, glycosylated phytase was isolated using degenerate oligodeoxyribonucleotides deduced from phytase amino acid (aa)sequences. Nucleotide (nt)sequence analysis of the cloned region revealed the presence of an open reading frame coding for 467 aa and interrupted once by an intron of 102 bp in the 5' part of the gene. The start codon is followed by a sequence coding for a putative signal peptide. Expression of phyA is controlled at the level of mRNA accumulation in response to inorganic phosphate levels. After cell growth in low-phosphate medium, a transcript of about 1.8 kb was visualized. Transcription of phyA initiates at at least seven start points within a region located 45-25 nt upstream from the start codon. In transformants of A. niger, expression of multiple copies of phyA resulted in up to more than tenfold higher phytase levels than in the wild-type strain. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Van Hartingsveldt, W. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Van Zeijl, C.M.J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hartevelda, G.M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Gouka, R.J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Suykerbuyk, E.M.G. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Luiten, R.G.M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Van Paridon, P.A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Van Paridon, P.A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Veenstra, A.E. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Van Gorcoma, R.F.M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Van Den Handel, C.A. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1_TWirIeavsVohaW2mkIhxBwDFKgzMVZv/view?usp=drivesdk">https://drive.google.com/file/d/1_TWirIeavsVohaW2mkIhxBwDFKgzMVZv/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-7127 | 25.06.2025 | 25.06.2025 | Documentos solicitados |