Nuclear DNA content of the whitefly Bemisia tabaci (Aleyrodidae: Hemiptera)estimated by flow cytometry (Record no. 41463)

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control field MX-MdCICY
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control field 20250625124655.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-7132
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Title Nuclear DNA content of the whitefly Bemisia tabaci (Aleyrodidae: Hemiptera)estimated by flow cytometry
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Volume/sequential designation Bulletin of Entomological Research, 95(4), p.309-312, 2005
520 3# - SUMMARY, ETC.
Summary, etc. The nuclear DNA content of the whitefly Bemisia tabaci (Gennnadius)was estimated using flow cytometry. Male and female nuclei were stained with propidium iodide and their DNA content was estimated using chicken red blood cells and Arabidopsis thaliana L. (Brassicaceae)as external standards. The estimated nuclear DNA content of male and female B. tabaci was 1.04 and 2.06 pg, respectively. These results corroborated previous reports based on chromosome counting, which showed that B. tabaci males are haploid and females are diploid. Conversion between DNA content and genome size (1 pg DNA = 980 Mbp)indicate that the haploid genome size of B. tabaci is 1020 Mbp, which is approximately five times the size of the genome of the fruitfly Drosophila melanogaster Meigen. These results provide an important baseline that will facilitate genomics-based research for the B. tabaci complex.
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Personal name Brown, J.K.
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Personal name Lambert, G.M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Ghanim, M.
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Personal name Czosnek, H.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Galbraith, D.W.
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Uniform Resource Identifier <a href="https://drive.google.com/file/d/1veDBPHX4BGbWZdYXU_BXxK718yFa9_tE/view?usp=drivesdk">https://drive.google.com/file/d/1veDBPHX4BGbWZdYXU_BXxK718yFa9_tE/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-7132 25.06.2025 25.06.2025 Documentos solicitados