mRNA Differential Display Visualized by Silver Staining Tested on Gene Expression in Resurrection Plant Boea hygrometrica1 (Record no. 41469)

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control field MX-MdCICY
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control field 20250625124655.0
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Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-7138
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245 10 - TITLE STATEMENT
Title mRNA Differential Display Visualized by Silver Staining Tested on Gene Expression in Resurrection Plant Boea hygrometrica1
490 0# - SERIES STATEMENT
Volume/sequential designation Plant Molecular Biology Reporter, 17(3), p.279, 1999
520 3# - SUMMARY, ETC.
Summary, etc. A new protocol for nonradioactive mRNA differential display with high sensitivity visualized by DNA silver staining is presented. The protocol has been tested on total RNA of resurrection plantBoea hygrometrica to identify genes differentially expressed in dehydrated leaves compared to fresh and re-hydrated leaves. Total RNAs were reversely transcribed using three anchored oligo-dT primers. PCR amplification of relative cDNAs was carried out in combination with eight arbitrary primers. Urea-denatured polyacrylamide gel electrophoresis is used to separate amplified products of various molecular weights. Bands of amplified transcripts were resolved by silver staining, and the sensitivity was high enough to detect the tiny differences in DNA electrophoresis pattern. Several specific transcripts were successfully identified by this procedure, and one of them was cloned and sequenced.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Deng, X.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Hu, Z.A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Wang, H.X.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1dBpCqhogKNRswle9TuHiwCdbfNPWkzA0/view?usp=drivesdk">https://drive.google.com/file/d/1dBpCqhogKNRswle9TuHiwCdbfNPWkzA0/view?usp=drivesdk</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-7138 25.06.2025 25.06.2025 Documentos solicitados