Electrophoretic karyotype and gene mapping of the vascular wilt fungus Verticillium dahliae (Record no. 41470)

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control field MX-MdCICY
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control field 20250625124655.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-7139
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245 10 - TITLE STATEMENT
Title Electrophoretic karyotype and gene mapping of the vascular wilt fungus Verticillium dahliae
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Volume/sequential designation FEMS MicroBiology Letters, 245(2), p.213-220, 2005
520 3# - SUMMARY, ETC.
Summary, etc. The karyotype profile of Verticillium dahliae was resolved by pulse-field gel lectrophoresis. It revealed 6 chromosomal bands that corresponded to 7 chromosomes as shown by RFLP analysis using as probe the telomeric consensus sequence (AACCCT)5. The number of chromosomes was further verified by the sensitivity of the hybridization signals to Bal31 digestion and the exclusion of interfering mitochondrial DNA signals. The corresponding sizes of the seven separated chromosomes were 6.7, 5.6, 4.1, 3.4, 3.1, 3.1 and 2.4 Mb, raising the total genomic size of the fungus to approximately 28.4 Mb. Twenty five homologous V. dahliae genes obtained either from randomly sequenced clones or PCR amplification were used as hybridization probes and were located onto the seven chromosomes.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Pantou, M.P.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Typas, M.A.
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Uniform Resource Identifier <a href="https://drive.google.com/file/d/16C3eg7asqAXN2PVLEaU-w5LRw7S074XC/view?usp=drivesdk">https://drive.google.com/file/d/16C3eg7asqAXN2PVLEaU-w5LRw7S074XC/view?usp=drivesdk</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-7139 25.06.2025 25.06.2025 Documentos solicitados