High throughput immuno-screening of cDNA expression libraries produced by in vitro recombination; exploring the Plasmodium falciparum proteome (Record no. 41489)

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fixed length control field 02176nam a2200301Ia 4500
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control field MX-MdCICY
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control field 20250625124656.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-7158
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Title High throughput immuno-screening of cDNA expression libraries produced by in vitro recombination; exploring the Plasmodium falciparum proteome
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Volume/sequential designation Molecular & BioChemical Parasitology, 133(2), p.267-274, 2004
520 3# - SUMMARY, ETC.
Summary, etc. Improved Plasmodium falciparum cDNA expression libraries were constructed by combining mRNA oligo-capping with in vitro recombination and directional cloning of cDNA inserts into a plasmid vector that expresses sequences as thioredoxin fusion proteins. A novel procedure has also been developed for the rapid identification of seropositive clones on high-density filters, using direct labelling of P. falciparum immune immunoglobulin with fluorescein isothiocynate (FITC). This approach combines the advantages of recombination-assisted cDNA cloning with high throughput, non-radioactive serological screening of expression libraries. Production of replicate colony matrices allows the identification of antigens recognised by different pools with different specificities from residents of a malaria endemic region. Analyses ofDNAsequences derived from sero-reactive colonies indicate that this is an effective method for producing recombinant proteins that react with antibodies from malaria-exposed individuals. This approach permits the systematic construction of a database of antigenic proteins recognised by sera from malaria-exposed individuals.
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Topical term or geographic name entry element PLASMODIUM FALCIPARUM
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Topical term or geographic name entry element CDNA
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Topical term or geographic name entry element IN VITRO RECOMBINATION
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Topical term or geographic name entry element PROTEIN MACRO-ARRAY
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kordai Sowa, M.P.
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Personal name Sharling, L.
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Personal name Humphreys, G.
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Personal name Cavanagh, D.R.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Gregory, W.F.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Fenn, K.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Creasey, A.M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Creasey, A.M.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/16z7CnuDjltYaDM9Y2rm17rgEvbwuFLUG/view?usp=drivesdk">https://drive.google.com/file/d/16z7CnuDjltYaDM9Y2rm17rgEvbwuFLUG/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-7158 25.06.2025 25.06.2025 Documentos solicitados