Cell wall composition and structure of Yarrowia lipolytica transposon mutants affected in calcofluor sensitivity (Record no. 41528)
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| fixed length control field | 02204nam a2200217Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625124656.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-7197 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Cell wall composition and structure of Yarrowia lipolytica transposon mutants affected in calcofluor sensitivity |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Antonie van Leeuwenhoek, 84(3), p.229-238, 2003 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | A collection of transposon-mutagenized strains of Yarrowia lipolytica was screened for wall defects by determination of their sensitivity to calcofluor white. A number of strains were hypersensitive, whereas others were resistant. Different non-allelic mutants displayed increased sensitivity to autolysis and lytic enzymes, independently of whether they were sensitive or resistant to calcofluor white. A thorough analysis of their cell walls revealed minor quantitative alterations, and no significant changes in chitin content. Electrophoretic analysis of wall-bound and excreted proteins proved to be a sensitive method that revealed defects in the cell wall structure of the mutants. Important alterations in the patterns of the wall proteins extracted by SDS or by enzymatic treatments were noticed for the mutants, as compared to the parental strain. Mutants released to the growth medium a larger number of protein species than the parental strain, suggesting impairment in wall assembly of certain polypeptides. Patterns of wall-bound and excreted proteins, as well as alterations in wall chemical composition were not diagnostic of calcofluor white sensitivity or resistance, but were specific for each mutant. Our data show that an increase in either sensitivity or resistance of Y. lipolytica to certain levels of calcofluor is equally indicative of alterations in cell wall structure, independent of chitin levels. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ruiz-Herrera, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | García-Maceira, P. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Castillo-Barahona, L.C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Valentín, E. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sentandreu, R. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/125GuTqmC3NHr8NTs7fG64mDP1ngWQl_2/view?usp=drivesdk">https://drive.google.com/file/d/125GuTqmC3NHr8NTs7fG64mDP1ngWQl_2/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-7197 | 25.06.2025 | 25.06.2025 | Documentos solicitados |