Screening for glycosylphosphatidylinositol (GPI)-dependent cell wall proteins in Saccharomyces cerevisiae (Record no. 41543)

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control field 20250625124657.0
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Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-7212
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245 10 - TITLE STATEMENT
Title Screening for glycosylphosphatidylinositol (GPI)-dependent cell wall proteins in Saccharomyces cerevisiae
490 0# - SERIES STATEMENT
Volume/sequential designation Mol. Gen. Genet., 258(1-2), p.53-59, 1998
520 3# - SUMMARY, ETC.
Summary, etc. Open reading frames in the genome of Saccharomyces cerevisiae were screened for potential glycosylphosphatidylinositol (GPI)-attached proteins. The identi®cation of putative GPI-attached proteins was based on three criteria: the presence of a GPI-attach-ment signal sequence, a signal sequence for secretion and a serine- or threonine-rich sequence. In all, 53 ORFs met these three criteria and 38 were further analyzed as follows. The sequence encoding the 40 C- terminal amino acids of each was fused with the struc- tural gene for a reporter protein consisting of a secretion signal, a-galactosidase and a hemagglutinin (HA)epitope, and examined for the ability to become incorpo- rated into the cell wall. On this basis, 14 of fusion proteins were classi®ed as GPI-dependent cell wall proteins because cells expressing these fusion proteins: (i)had high levels of a-galactosidase activity on their surface; (ii)released signi®cant amounts of the fusion proteins from the membrane on treatment with phosphatidylinositol-speci®c phospholipase C (PI-PLC); and (iii)released fusion proteins from the cell wall following treatment with laminarinase. Of the 14 identi®ed putative GPI-dependent cell wall proteins, 12 had novel ORFs adjacent to their GPI-attachment signal sequence. Amino acid sequence alignment of the C-terminal sequences of the 12 ORFs, together with those of known cell wall proteins, reveals some sequence similarities among them.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SACCHAROMYCES CEREVISIAE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element YEAST
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CELL WALL PROTEIN
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element GPI-ANCHORED PROTEIN
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Hamada, K.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Fukuchi, S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Arisawa, M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Baba, M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kitada, K.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1y2RnaIWWiOAzhndc2QbfqM9nwbN_kP6h/view?usp=drivesdk">https://drive.google.com/file/d/1y2RnaIWWiOAzhndc2QbfqM9nwbN_kP6h/view?usp=drivesdk</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-7212 25.06.2025 25.06.2025 Documentos solicitados