Specificity of Amaranthus leucocarpus syn. hypocondriacus lectin for O-glycopeptides (Record no. 41544)

MARC details
000 -LEADER
fixed length control field 02280nam a2200313Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625124657.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-7213
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fixed length control field 250602s9999 xx |||||s2 |||| ||und|d
245 10 - TITLE STATEMENT
Title Specificity of Amaranthus leucocarpus syn. hypocondriacus lectin for O-glycopeptides
490 0# - SERIES STATEMENT
Volume/sequential designation Biochimica et Biophysica Acta, 1674(3), p.282-290, 2004
520 3# - SUMMARY, ETC.
Summary, etc. this work, we determined a value of 1.0-10-2 M for the association constant of ALL for GalNAc, calculated using fluorescence spectroscopy assays. Using neoglycopeptides obtained by in vitro O-glycosylation, we determined the main features of O-glycopeptides recognized by ALL using molecular dynamics simulations, capillary electrophoresis, and ELISA. Neo-glycopeptides were obtained by in vitro O-glycosylation reaction using microsomal preparations of murine thymocytes, human gastric fundus and colonic mucosa. ELISA assays were performed with peroxidase-labeled murine monoclonal IgG2, n light chain (5D4)antibodies against ALL. Among the in vitro neoglycopeptides, only those of TTSAPTTS containing GalNAc at Thr in #2 and #6 reacted with ALL. Neither the TTSAPTTS glycopeptide, containing a unique GalNAc residue at Thr in #2, nor others (with more than two GalNAc residues)interacted with the lectin. Computational docking assays of the lower energy conformers for interactions between glycopeptides and lectins confirmed that ALL recognized GalNAc residues when they are spaced out in glycan structures, whereas GalNAc residues arranged in clusters prevented interaction with the lectin, indicating that ALL is specific for a special GalNAc-containing motif found in different O-glycoproteins.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element AMARANTHUS LEUCOCARPUS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PLANT LECTIN
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element T- AND TN-SPECIFIC LECTIN
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element O-GLYCAN
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Hernández, P.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Tetaert, D.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Vergoten, G.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Debray, H.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Jimenez, M.C.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Alvarez, G.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Agundis, C.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Agundis, C.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Zenteno, E.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1yIkayHCeGjdY6_77mjwhinrcI0TXiMEK/view?usp=drivesdk">https://drive.google.com/file/d/1yIkayHCeGjdY6_77mjwhinrcI0TXiMEK/view?usp=drivesdk</a>
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942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-7213 25.06.2025 25.06.2025 Documentos solicitados