Characterisation of CwpA, a putative glycosylphosphatidylinositolanchored cell wall mannoprotein in the Wlamentous fungus Aspergillus niger (Record no. 41593)

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fixed length control field 02079nam a2200313Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625124657.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-7262
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245 10 - TITLE STATEMENT
Title Characterisation of CwpA, a putative glycosylphosphatidylinositolanchored cell wall mannoprotein in the Wlamentous fungus Aspergillus niger
490 0# - SERIES STATEMENT
Volume/sequential designation Fungal Genetics and Biology, 42(10), p.873-885, 2005
520 3# - SUMMARY, ETC.
Summary, etc. Glycosylphosphatidylinositol (GPI)-anchored proteins in fungi are found at the cell surface, either as plasma membrane proteins (GPI-PMPs)or attached by a remnant of the GPI-anchor to the cell wall (GPI-CWPs). GPI-CWPs can be extracted from the cell wall by treatment with hydroXuoric acid (HF), which cleaves the phosphodiester bond that is present in the remnant of the GPIanchor. The Wlamentous fungus Aspergillus niger contains at least seven HF-extractable cell wall mannoproteins. One gene encoding an HF-extractable cell wall mannoprotein, cwpA, was cloned and further characterised. The protein sequence of CwpA indicated the presence of two hydrophobic signal sequences both at the N-terminus and C-terminus of the protein, for entering the ER and the addition of a GPI-anchor, respectively. A CwpA-speciWc antiserum was raised and in combination with fractionation experiments, we show that this protein was abundantly present as an HF-extractable protein in the cell wall of A. niger. ÆÉ 2005 Elsevier Inc. All rights reserved.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CELL WALL INTEGRITY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element GPI-ANCHORING
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CALCOXUOR WHITE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element HYPERSENSITIVE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element HF-EXTRACTIONS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element BETA-SITE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element GLUCAN
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Damveld, R.A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Arentshorst, M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Vankuyk, P.A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Klis, F.M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Van Den Hondel, C.A.M.J.J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Rama, A.F.J.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1k5V9oc2XeVMCipISDjokYD5tfKRT_Omb/view?usp=drivesdk">https://drive.google.com/file/d/1k5V9oc2XeVMCipISDjokYD5tfKRT_Omb/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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