Import of polyphenol oxidase by chloroplasts is enhanced by methyl jasmonate (Record no. 41607)

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control field MX-MdCICY
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control field 20250625124658.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-7276
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Title Import of polyphenol oxidase by chloroplasts is enhanced by methyl jasmonate
490 0# - SERIES STATEMENT
Volume/sequential designation Planta, 219(3), p.412-419, 2004
520 3# - SUMMARY, ETC.
Summary, etc. Polyphenol oxidase (PPO; EC 1.10.3.2 or EC 1.14.18.1)takes part in the response of tomato plants (Lycopersicon esculentum Mill.)to wounding and herbivore attack, mediated by the octadecanoid oundsignaling pathway. Wounding and methyl jasmonate (MeJA)induce expression of ppo genes and markedly increase the level of the enzyme. We report that pretreatment with MeJA also markedly increased the ability of isolated tomato chloroplasts to import and process PPO precursors (pPPO). Pea (Pisum sativum L.)chloroplasts showed no such response. Wounding or ethylene alone was ineffective but ethylene was synergistic with MeJA. Treatment with MeJA conferred a strong binding of pPPO, or its processing intermediate, to thylakoids and subsequent translocation into the lumen and processing to the mature protein. The effect on PPO import and translocation was evident after 8-16 h exposure to MeJA. Membrane-bound pPPO was crosslinked to a proteinaceous component of the thylakoid translocation apparatus, apparently induced by MeJA. The import and processing of other nuclear-encoded thylakoid proteins were not affected by MeJA in tomato. A 90-kDa protein that co-fractionated with thylakoids was induced along with the increase in competence for PPO import, and was identified as the proteinaseinhibitor multicystatin. It is concluded that the 90-kDa protein observed is part of the MeJA-induced defense
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Koussevitzky, S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Ne'Eman, E.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Harel, E.
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Uniform Resource Identifier <a href="https://drive.google.com/file/d/1wqUJkyM9OWvZSRsj6XhIhJt_lnO6w_m1/view?usp=drivesdk">https://drive.google.com/file/d/1wqUJkyM9OWvZSRsj6XhIhJt_lnO6w_m1/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-7276 25.06.2025 25.06.2025 Documentos solicitados