Cloning and Characterization of Red Clover Polyphenol Oxidase cDNAs and Expression of Active Protein in Escherichia coli and Transgenic Alfalfa1[w] (Record no. 41608)

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control field MX-MdCICY
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control field 20250625124658.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-7277
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Title Cloning and Characterization of Red Clover Polyphenol Oxidase cDNAs and Expression of Active Protein in Escherichia coli and Transgenic Alfalfa1[w]
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Volume/sequential designation Plant Physiology, 136(2), p.3234-3244, 2004
520 3# - SUMMARY, ETC.
Summary, etc. Red clover (Trifolium pratense)leaves contain high levels of polyphenol oxidase (PPO)activity and o-diphenol substrates. Wounding of leaves during harvest and ensiling results in browning of leaf tissues from activity of PPO on the o-diphenols. In association with browning, leaf proteins remain undegraded during ensiling, presumably due to PPO-generated o-quinone inhibition of leaf proteases. We cloned three red clover PPO cDNAs, PPO1, PPO2, and PPO3, from a leaf cDNA library. Sequence comparisons among the three red clover PPO clones indicated they are 87
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Sullivan, M.L.
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Personal name Hatfield, R.D.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Thoma, S.L.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Samac, D.A.
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Uniform Resource Identifier <a href="https://drive.google.com/file/d/18xfyKpHNCv7uJN8mXdO4CzvUs6dR5nMV/view?usp=drivesdk">https://drive.google.com/file/d/18xfyKpHNCv7uJN8mXdO4CzvUs6dR5nMV/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-7277 25.06.2025 25.06.2025 Documentos solicitados