MARC details
| 000 -LEADER |
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| fixed length control field |
02055nam a2200241Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625124658.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-7279 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Purification and characterization of polyphenol oxidase from nettle (Urtica dioica L.)and inhibitory effects of some chemicals on enzyme activity |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Journal of Enzyme Inhibition and Medicinal Chemistry, 20(3), p.297-302, 2005 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Polyphenol oxidase (PPO)of nettle (Urtica dioica L.)was extracted and purified through (NH4)2SO4 precipitation, dialysis, and CM-Sephadex ion-exchange chromatography and was used for its characterization. The PPO showed activity to catechol, 4-methylcatechol, L-3,4-dihydroxyphenylalanine (L-DOPA), L-tyrosine, p-cresol, pyrogallol, catechin and trans-cinnamic acid. For each of these eight substrates, optimum conditions such as pH and temperature were determined and L-tyrosine was found to be one of the most suitable substrates. Optimum pH and temperature were found at pH 4.5 and 308C respectively and Km and Vmax values were 7.90 £ 1024 M, and 11290 EU/mL for with L-tyrosine as substrate. The inhibitory effect of several inhibitors, L-cysteine chloride, sodium azide, sodium cyanide, benzoic acid, salicylic acid, L-ascorbic acid, glutathione, thiourea, sodium diethyl dithiocarbamate, b-mercaptoethanol and sodium metabisulfite were tested. The most effective was found to be sodium diethyl dithiocarbamate which acted as a competitive inhibitor with a Ki value of 1.79 £ 1029 M. In addition one isoenzyme of PPO was detected by native polacrylamide slab gel electrophoresis. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
POLYPHENOL OXIDASE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
ENZYME |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
NETTLE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
URTICA DIOICA L |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Gulcin, I. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Kufrevioglu, O.I. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Oktay, M. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1RXwp3g5oBGmGiQlbz-I0ayknXRTRyyhk/view?usp=drivesdk">https://drive.google.com/file/d/1RXwp3g5oBGmGiQlbz-I0ayknXRTRyyhk/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
