A comparative assessment of DNA fingerprinting techniques (RAPD, ISSR, AFLP and SSR)in tetraploid potato (Solanum tuberosum L.)germplasm (Record no. 41691)

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control field 20250625124659.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-7360
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Title A comparative assessment of DNA fingerprinting techniques (RAPD, ISSR, AFLP and SSR)in tetraploid potato (Solanum tuberosum L.)germplasm
490 0# - SERIES STATEMENT
Volume/sequential designation Euphytica, 113, p.135-144, 2000
520 3# - SUMMARY, ETC.
Summary, etc. Several DNA marker systems and associated techniques are available today for fingerprinting plant germplasm but information on their relative usefulness in particular crops is limited. The study investigated PCR based DNA fingerprinting in a set of 39 potato cultivars using RAPDs (20 primers), ISSRs (6 primers), AFLPs (2 primers)and SSRs (5 primer pairs). Results show that each of the four techniques can on their own, individually identify each cultivar, but that techniques differ in the mean number of profiles generated per primer (or primer pair)per cultivar, referred to as Genotype Index (GI). The order of merit based on this criterium and in this material was AFLPs (GI = 1.0), a multi-locus SSR (GI = 0.77), RAPDs (GI = 0.53), ISSRs (GI = 0.47)and single locus SSRs (GI = 0.36). Problems in relating banding patterns to individual loci and alleles for polyploid genomes, using these techniques as they are currently employed, are also discussed.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name McGregor, C.E.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Lambert, C.A.
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Personal name Greyling, M.M.
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Personal name Louw, J.H.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Warnich, L.
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Uniform Resource Identifier <a href="https://drive.google.com/file/d/1l7DXflw0K7E7G3Ow-3I1KMsCP3oQFOkY/view?usp=drivesdk">https://drive.google.com/file/d/1l7DXflw0K7E7G3Ow-3I1KMsCP3oQFOkY/view?usp=drivesdk</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-7360 25.06.2025 25.06.2025 Documentos solicitados