Phosphoproteome Analysis of Capacitated Human Sperm (Record no. 41713)
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| fixed length control field | 02720nam a2200289Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625124700.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-7382 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Phosphoproteome Analysis of Capacitated Human Sperm |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | The Journal of Biological Chemistry, 278(13), p.11579-11589, 2003 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Before fertilization can occur, mammalian sperm must undergo capacitation, a process that requires a cyclic AMP-dependent increase in tyrosine phosphorylation. To identify proteins phosphorylated during capacitation, two-dimensional gel analysis coupled to antiphosphotyrosine immunoblots and tandem mass spectrometry (MS/MS)was performed. Among the protein targets, valosin-containing protein (VCP), a homolog of the SNARE-interacting protein NSF, and two members of the A kinase-anchoring protein (AKAP)family were found to be tyrosine phosphorylated during capacitation. In addition, immobilized metal affinity chromatography was used to investigate phosphorylation sites in whole protein digests from capacitated human sperm. To increase this chromatographic selectivity for phosphopeptides, acidic residues in peptide digests were converted to their respective methyl esters before affinity chromatography. More than 60 phosphorylated sequences were then mapped by MS/MS, including precise sites of tyrosine and serine phosphorylation of the sperm tail proteins AKAP-3 and AKAP-4. Moreover, differential isotopic labeling was developed to quantify phosphorylation changes occurring during capacitation.The phosphopeptide enrichment and quantification methodology coupled to MS/MS, described here for the first time, can be employed to map and compare phosphorylation sites involved in multiple cellular processes. Although we were unable to determine the exact site of phosphorylation of VCP, we did confirm, using a cross-immunoprecipitation approach, that this protein is tyrosine phosphorylated during capacitation. Immunolocalization of VCP showed fluorescent staining in the neck of noncapacitated sperm. However, after capacitation, staining in the neck decreased, and most of the sperm showed fluorescent staining in the anterior head. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ficarro, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Chertihin, O. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Westbrook, V.A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | White, F. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Jayes, F. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kalab, F. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Marto, J.A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Marto, J.A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Herr, J.C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hunt, D.F. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Visconti, P.E. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1b-2aB5OQQv0U_OtDKBfMOxk-mWfLRKoJ/view?usp=drivesdk">https://drive.google.com/file/d/1b-2aB5OQQv0U_OtDKBfMOxk-mWfLRKoJ/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-7382 | 25.06.2025 | 25.06.2025 | Documentos solicitados |