RAPD, ISSR and RFLP fingerprints as useful markers to evaluate genetic integrity of micropropagated plants of three diploid and triploid elite tea clones representing Camellia sinensis (China type)and C. assamica ssp. assamica (Assam-India type) (Record no. 41720)

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control field 20250625124700.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-7389
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245 10 - TITLE STATEMENT
Title RAPD, ISSR and RFLP fingerprints as useful markers to evaluate genetic integrity of micropropagated plants of three diploid and triploid elite tea clones representing Camellia sinensis (China type)and C. assamica ssp. assamica (Assam-India type)
490 0# - SERIES STATEMENT
Volume/sequential designation Plant Cell Reports, 21, p.166-173, 2002
520 3# - SUMMARY, ETC.
Summary, etc. An efficient in vitro propagation method using enhanced axillary branching cultures produced plants from nodal explants of three mature, elite tea clones: diploid UPASI 26 and UPASI 27 (2n=2x=30)representing Camellia sinensis (China type)and triploid UPASI 3 (2n=3x=45)representing C. assamica ssp. assamica (Assam- India type). The genetic fidelity of the micropropagated plants of these three tea clones was assessed by analysing their nuclear, mitochondrial (mt), and chloroplast (cp)genomes using multiple molecular DNA markers. A total of 465, 446 and 462 genetic loci were produced with RFLP, RAPD and ISSR fingerprinting in the micropropagated plants and the corresponding mother plant of C. sinensis clone U (UPASI)26, and C. assamica ssp. assamica clones U3 and U27, respectively. RFLP fingerprinting was performed using six restriction endonuclease digests and 14 mt and cp gene probes in 84 enzymeprobe combinations. For PCR fingerprinting, 50 RAPD and SSR primers were used for amplifications. The micropropagated plants of both the U3 and U27 clones revealed complete stability in the 462 and 446 genetic loci analysed. In comparison, 36 (7.7
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CAMELLIA SINENSIS (CHINA TYPE)
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CAMELLIA ASSAMICA SSP. ASSAMICA (ASSAM-INDIA TYPE)
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ENHANCED AXILLARY BRANCHING
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MOLECULAR DNA MARKERS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SOMACLONAL VARIATION
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Devarumath, R. M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Nandy, S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Rani, V.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Marimuthu, S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Muraleedharan, N.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Raina, S. N.
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Uniform Resource Identifier <a href="https://drive.google.com/file/d/14jsJBrBeW_B8fhuBQe0RI8JzMKssLhSS/view?usp=drivesdk">https://drive.google.com/file/d/14jsJBrBeW_B8fhuBQe0RI8JzMKssLhSS/view?usp=drivesdk</a>
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