A novel TMV-induced hot pepper cell wall protein gene (CaTin2)is associated with virus-specific hypersensitive response pathway (Record no. 41725)

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fixed length control field 02427nam a2200277Ia 4500
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control field MX-MdCICY
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control field 20250625124700.0
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Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-7394
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245 10 - TITLE STATEMENT
Title A novel TMV-induced hot pepper cell wall protein gene (CaTin2)is associated with virus-specific hypersensitive response pathway
490 0# - SERIES STATEMENT
Volume/sequential designation Plant Molecular Biology, 51, p.687-701, 2003
520 3# - SUMMARY, ETC.
Summary, etc. Incompatible plant-pathogen interactions result in the rapid cell death response known as hypersensitive response (HR)and activation of host defense related genes. To understand the cellular mechanism controlling defense response better, a novel pathogenesis-related (PR)gene and putative cell wall protein gene, CaTin2, was isolated through differential screening of a hot pepper cDNA library and characterized. CaTin2 gene was locally and systemically induced in hot pepper plants upon TMV-P0 inoculation which induces HR. However, CaTin2 gene wasn't regulated by bacterial HR-specific signal pathway. The full-length cDNA for CaTin2, which is 864 nucleotides long, contained the open reading frame of 200 amino acids including cell wall targeting sequences of 26 amino acids. CaTin2 gene has no sequence similarity with other cell wall protein genes except the signal sequence and exists as only one copy in hot pepper genome. CaTin2 gene contains repeated helix-turn-helix motif consisting of 39 amino acids. CaTin2 mRNA accumulation was induced in response to various treatments such as ethylene, SA, MeJA, ABA, methyl viologen, NaCl and wounding at early time points. Subcelluar localization of CaTin2 was confirmed in the cell wall in hot pepper leaves by making CaTin2::smGFP fusion protein. The transgenic plants overexpressing CaTin2 cDNA were resistant to TMV and CMV inoculation. From these results, CaTin2 gene may encode a virus-related new cell wall protein member.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CDNA CLONING
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CELL WALL PROTEIN
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DIFFERENTIAL SCREENING
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element HOT PEPPER (CAPSICUM ANNUUM L.)
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element HYPERSENSITIVE RESPONSE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TOBACCO MOSAIC VIRUS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Shin, R.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Park, C-J
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name An, J-M
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Paek, K-H
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1MKckESvWzphpL4rCAwKGoBFPvu7EjWat/view?usp=drivesdk">https://drive.google.com/file/d/1MKckESvWzphpL4rCAwKGoBFPvu7EjWat/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-7394 25.06.2025 25.06.2025 Documentos solicitados